Efficacy of Clinical Application of Phosphatidylcholine Liposomes: Comparative Ultrastructural Analysis of Gingival Epithelium at Different Stages of Treatment of Patients with Generalized Periodontitis


generalized periodontitis
ultrastructural morphology


On the basis of comparative ultrastructural analysis of the gingival mucosa (GM) before and during the stages of clinical application of phosphatidylcholine liposome there was revealed that the basal layer of the epithelium was relatively expanded and represented by cells that preserved the structure typical for normal mucous membrane. It indicated an increase in reparative potential of this layer filled with cambial cells to restore epithelial integrity. In the stratum spinosum cells were more distinctly joined to each other with desmosomes that provided the integrity of the connections between cells and maintenance of barrier properties of the epithelium; in the stratum granulosum complete recovery of cells was not observed, however, the structure of most keratinocytes resembled the normal one. The efficacy of using advanced therapeutic dental complex (TDC) (deposition of egg phosphatidylcholine liposomes (EPCL) by means of infiltrative injections and periodontal bandage) was also indicated by an increase in functional activity of gingival epithelial cells: the presence of large functional nucleoli in the nuclei of euchromatin which predominates over heterochromatin reflecting the activation of the RNA and protein biosynthesis on the background of the expansion of the nuclear pores as morphological equivalent of the intensification of the nuclear-cytoplasmic transport process. 


Antonov SV. Dentistry: regulatory legal acts and documents. Tsentr medychnoho prava. Kyiv. 2009;696.

Babay OM, Krasnopolskyi YuM. Efficacy of using “Lipin” in complex treatment of generalized periodontitis. Stomatoloh. 2003;5:32-38.

Grigoryan AS, Grudyanov AI. Key elements of the pathogenesis of periodontal diseases with consideration to cytomorphometric method of the research. Stomatologiya. 2001;1:5-9.

Kaplun AP, Shon Le Bang, Krasnopolskyi YuM. Liposomes and other nanoparticles as a means of drug delivery. VMKh. 1999;3:3-13.

Kylymnychenko OI. Usage of lipin in complex treatment of hypoxia of different etiology [dissertation]. Donetsk National Medical University. Dnipropetrovsk. 1998;17.

Kunin AA, Ippolitov YuA, Lepekhina LI, Bykov EG. Clinical histochemistry of the barrier function of gingival mucosa in periodontitis. Stomatologiya. 2001;1:13-16.

Kushnirenko S. Properties of liposomes and their use in clinical medicine. Liky Ukraiiny. 2003;1:18-20.

Leontyev VK. Healthy teeth and quality of life. Stomatologiya dlya vsekh. 1999;2(3):30-34.

Babay OM, Krasnopolskyi YuM, Babay AM, inventors; Babay OM, Krasnopolskyi YuM, Babay AM, assignee. Method of treatment of inflammatory and inflammatory-destructive processes in paradontium. Ukraine’s patent UA 55454. 2003 Apr. 15.

Perova HI. Experimental and clinical substantiation of using the lecithin-antioxidant complex in treatment of generalized periodontitis [dissertation]. Odesa National Medical University. Odesa. 2002;21.

Babay ОM, Merkulova UV, Deieva TV. Comparative clinical and morphological analysis of the periodontitis of different genesis. Eksperymentalna ta klinichna medytsyna. 2015;2(67):60-63.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.


Download data is not yet available.