AbstractThe objective of the research was to determine antiradical, antimetastatic and antitumor activity of kaolin preparation “Kremnevit”.Material and methods. Experiments were carried out on 30 male C57BL mice inoculated with Lewis lung carcinoma (LLC) cells. The rate of superoxide radical generation in tumor cells as well as in animal organs was determined using 1-hydroxy-2,2,6,6-tetramethyl-4-oxopyrrolidin- hydrochloride spin traps and electron spin resonance (ESR) spectroscopy at room temperature.Results of the experiments showed that the preparation “Kremnevit” exhibits antitumor activity reducing the tumor mass by 24% in experimental animals compared to animals which did not receive the preparation. “Kremnevit” affects the rate of superoxide radical generation by intracellular (mitochondria) and extracellular sources (the NADPH oxidase of tumor-associated neutrophils (TANs). The superoxide-generating activity of TANs increased in mice treated with “Kremnevit” compared to animals which did not receive it.Conclusions. Non-toxic kaolin preparation “Kremnevit” has antitumor and antimetastatic activity regulating electron transport in hepatocyte mitochondria during superoxide radical generation by hepatocyte mitochondria and immunocompetent blood cells of mice inoculated with LLC cells.
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