Influence of Extracellular Dehydration on Histomorphometrical Parameters of Rats’ Spleen
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Keywords

spleen
white pulp
morphometry
dehydration

Abstract

The article presents the data obtained during the analysis of structural components of the spleen under the conditions of extracellular dehydration of the experimental rats. The extracellular dehydration was created by giving the animals water with a diuretic (LASIX®). In order to study the structural components of the spleen histological sections were stained with hematoxylin-eosin and according to van Gieson's stain. Nonspecific dystrophic-degenerative and discirculatory processes appeared in the spleen, both during the mild and the moderate degree of dehydration. They manifested in delymphatisation of the white pulp and reduction of red and white pulp. According to morphometry, there was reduction of white pulp area on background of increase in red pulp area and connective tissue component at both terms of the experiments. Pearson's chi-square test and Kolmogorov-Smirnov test indicated that the change of the measured spleen parameters had approximately the same meaning both after one and two months of extracellular dehydration, intensity of the decrease in the per cent of the white pulp area and the increase in the per cent of the red pulp occurred both after the first month and after the second month of the experiment, the most intensive increase in the connective tissue component was observed after two months of the experiment.
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