AbstractThe aim of the study was to investigate the dynamics of ultrastructural changes in pulmonary hemocapillaries during experimental diabetes mellitus (DM). Experiments were performed on 40 white male rats Wistar. In the experimental group, diabetes was modeled by intraperitoneal streptozotocin administration, firm «Sigma» (USA), diluted in 0.1 M citrate buffer, pH 4.5, at the rate of 60 mg/kg. Development of the disease was monitored by the glucose levels growth in the animals’ blood. Collecting of lung tissue for electron microscopic examination was performed under general ketamine anesthesia after 1, 2 and 4 weeks after administration of streptozotocin. During the first 2 weeks of the study in the cytoplasm of pulmonary hemocapillaries endothelial was growing the number of micropinocytose vesicules, edema and expansion components of the Golgi complex and granular endoplasmic grid. There can be found erythrocytic sludges in some hemocapillaries. It was found that the most expressed changes in the hemomicrocirculatory channels appear 4 weeks after modeling diabetes. In the endotheliocyte of the pulmonary hemocapillaries there were observed vacuolation of the cytoplasm, swelling of cytoplasmic organelles, thickening of the basement membrane, adhesion and aggregation of blood cells. Thus, studies have shown that experimental diabetes is accompanied by ultrastructural changes of pulmonary hemomicrocirculatory channels components, the severity of which depends on the streptozotocin-induced DM progress.
Barinova M.E., Kanana N.M., Sulayeva O.M. INOS activity of peripheral blood monocytes in the dynamics of experimental diabetes in rats with different sensitivity of AT1 receptor. Zdobutky klinichnoii i eksperymentalnoii medytsyny. 2008; 2: 27-29.
Borys R.Ya. Electron microscopic examination of the hemomictocirculatory channel in different layers of the skin of white rats during experimental diabetes. Visnyk morfolohiii. 2010; 16(1): 63-66.
Dzhalilova E.A., Kryvko Yu.A. Ultrastructural characteristics of the capillary level of the left part of rat’s heart in normal and in early streptozotocin-induced diabetes. Halytskyi likarskyi visnyk. 2010; 17(2): 51-53.
Demidov V.M., Lupanov K.V., Moskalova S.V., Rozumna Ye.M. Integrated pathogenic therapy of diabetic peripheral polyneuropathy in rats in experiment. Eksperymentalna ta klinichna fiziolohiia i biolohiia. 2003; 2(22): 67-71.
Zhurakivska O.Ya. Ultrastructural changes in the hemomictocirculatory channel of pituitary gland during the early stages of diabetes. Halytskyi likarskyi visnyk. 2010; 17(2): 28-30.
Kryvko Yu.A., Mateshuk-Vatseba L.R., Masna Z.Z. Ultrastructure links of the hemomictocirculatory channels in normal and in experimental diabetes. Visnyk morfolohiii. 2010; 16(2); 397- 400.
Meretskyi V.M. Features of the free radical lipid peroxidation and antioxidant protection in tissues of the kidney and liver in experimental diabetes. Zdobutky klinichnoii i eksperymentalnoii medytsyny. 2012; 1: 96-98.
Rodynskyi O.H., Huz V.A. Mechanism of damage to the central and peripheral nervous system in experimental diabetes. Medychni perspektyvy. 2009; 3: 4-14.
Leibson C.L. Ranson J.E., Olsen W., Zimmerlan B.R. Peripheral artery disease, diabetes, and mortality. J. Intern. Med. 2003; 253: 574-581.
Litonjua A., Lazarus R., Sparrow D., Demolles D., Weiss S. Lung function in type 2 diabetes: the Normative Aging Study. Respir. Med. 2005; 12: 1583-1590.
Potenza M.A., Gagliardi S., Nacci C. Endothelial dysfunction in diabetes: from mechanisms to therapeutic targets. Curr. Med. Chem. 2009; 16 (1): 94-112.
Sahebjami Н., Denholm D. Effects of streptozotocin-induced diabetes on lung mechanics and biochemistry in rats. Journal of Applied Physiology. 2003; 64 (1): 147-153.
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