Estimation of iron nanoparticles’ substance biosafety in vitro and in vivo

L. S. Rieznichenko, A. M. Doroshenko, S. M. Dybkova, T. G. Gruzina, Z. R. Ulberg, I. S. Chekman


The aim of the work was to estimate biosafety of iron nanoparticles’ substance according to in vitro and in vivo tests. Materials and Methods: biosafety of the substance of 40 nm sized spherical zero-valent iron nanoparticles has been established in vitro using cytotoxicity and genotoxicity tests. Biosafety in vivo has been determined according to the acute toxicity parameter (LD50) and the effect on cardiac function and state of rabbits’ hemodynamics. Results: there were no cytotoxic and genotoxic effects of iron nanoparticles’ substance on the test culture of eukaryotic cells. In case of intravenous injection of nanoparticles the average LD50 for BALB/с mice was 220.3 mg/kg. Iron nanoparticles’ substance injected intravenously was safe according to hemodynamic parameters. Conclusion: obtained data on biosafety estimation denotes low level of potential risks associated with iron nanoparticles’ substance.


iron nanoparticles; biosafety; genotoxicity; cytotoxicity; hemodynamics

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Copyright (c) 2017 L. S. Rieznichenko, A. M. Doroshenko, S. M. Dybkova, T. G. Gruzina, Z. R. Ulberg, I. S. Chekman

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