AbstractOne of the most important pathogenetic mechanisms of pregnancy complications are disregulation changes caused by the functioning disturbance of the nitric oxide depending (nitrergic) systems. Hypoxia occuring in iron deficiency anemia affects the activity of guanylate cyclase, which is an oxygen acceptor and intimately depends on the nitric oxide production. Thus, iron deficiency acts as a trigger for mediation violation of nitrergic system.The objective of the study was to evaluate the expression of endothelial nitric oxide synthase (eNOS) in placental tissue of the patients with iron deficiency anemia.The research was conducted at the maternity home № 2 (Odessa) and pathomorphology department of SI "IPAG NAMSU", Kyiv). 20 pregnant women were examined. They were divided into two groups: I (control group) included10 women with physiological pregnancy and childbirth and II (experimental group) included 10 women suffering from iron deficiency anemia during pregnancy. The level of eNOS expression was assessed with the use of immunohistochemical method.The expression of eNOS in placental tissue of women giving birth who had signs of iron deficiency anemia during the whole period of gestation, decreases in both the cytoplasm of syncytium villi and endothelium of fetal capillaries, and cytoplasm of vascular endothelial decidua. The described changes in the expression of eNOS may act as prerequisites for the development of endothelial dysfunction and associated pathological conditions during pregnancy.
Ancheva I.A. Clinical epidemiology of pregnancy anemia in the Southern Ukraine: reptrospective study. Visnyk problem biologii i medytzyny. 2013; 2(3): 112-114
MHU Order № 782 issued on 29.12.2005 About approval of clinical protocols for obstetric and gynecological care. Retrieved from: www.moz.gov.ua
Reutov V.P., Gozhenko A.I., Sorokina E.G., Nasibullin B.A., Babienko V.V., Kositzyn N.S. The problems of nitric oxide and cyclicity in biology and medicine. 2005; 105.
Semenova M.V., Bazhenov E.L., Kanunnikova N.M., Terekhova T.V. The state of placenta in iron deficiency anemia in pregnant women. Morphologicheskiie vedomosti. 2007; 1 (1-2): 218-219.
Trakhtenberg I.M., BabienkoV.V. Environmental-hygienic and toxic issues of the nitric oxide and its precursors’ effects on the organism. Toxicologicheskiy vestnik. 2013; 6 (123): 6-10.
Yakubova E.G., Suplotova L.A., Kukarskaya I.I. The impact of iron deficiency anemia on the perinatal outcomes in the population of pregnant women. Meditzinskaya nauka i obrazovanie Urala. 2010; 11 (3): 158-159.
Ahmed T., Hossain M., Sanin KI. Global burden of maternal and child undernutrition and micronutrient deficiencies. Ann Nutr Metab. 2012; 61(1): 8-17
Haider BA, Olofin I, Wang M, Spiegelman D, Ezzati M, Fawzi W; Nutrition Impact Model Study Group (anaemia). Anaemia, prenatal iron use, and risk of adverse pregnancy outcomes: systematic review and meta-analysis. BMJ. 2013; 346: f3443
Pasricha SR, Drakesmith H, Black J, Hipgrave D, Biggs BA. Control of iron deficiency anemia in low- and middle-income countries. Blood. 2013; 121(14): 2607-17
Ignarro L.J.Nitric Oxide: Biology and Pathobiology NY., Academic Press; 2 ed. 2009: 845.
Immunohistochemistry: Basics and Methodsed. Igor B. Buchwalow, Werner Böcker N.Y., Springer; 2010: 156.
Miller JL. Iron deficiency anemia: a common and curable disease. Cold Spring Harb Perspect Med. 2013; 3(7). a011866
By K, Qaqish BF, Preisser JS, Perin J, Zink RC. ORTH: R and SAS software for regression models of correlated binary data based on orthogonalized residuals and alternating logistic regressions. Comput Methods Programs Biomed. 2014; 113(2): 557-68
Orange SJ, Painter D, Horvath J, Yu B, Trent R, Hennessy A. Placental endothelial nitric oxide synthase localization and expression in normal human pregnancy and pre-eclampsia. Clin Exp Pharmacol Physiol. 2003; 30(5-6): 376-81
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